RNA folding in living cells
نویسندگان
چکیده
منابع مشابه
Temperature dependence of protein folding kinetics in living cells.
We measure the stability and folding rate of a mutant of the enzyme phosphoglycerate kinase (PGK) inside bone tissue cells as a function of temperature from 38 to 48 °C. To facilitate measurement in individual living cells, we developed a rapid laser temperature stepping method capable of measuring complete thermal melts and kinetic traces in about two min. We find that this method yields impro...
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ژورنال
عنوان ژورنال: RNA Biology
سال: 2010
ISSN: 1547-6286,1555-8584
DOI: 10.4161/rna.7.6.13554